2GSF
The Human Epha3 Receptor Tyrosine Kinase and Juxtamembrane Region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-24 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.163, 38.274, 76.333 |
| Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.770 |
| R-factor | 0.15996 |
| Rwork | 0.158 |
| R-free | 0.19455 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.415 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.038 | 0.099 |
| Number of reflections | 29945 | |
| <I/σ(I)> | 48.8 | 21.6 |
| Completeness [%] | 95.8 | 64.5 |
| Redundancy | 6.8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 20 mg/mL protein, 25% PEG 3350, 0.2M Ammonium Sulfate, 0.1M Hepes, 20% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K, pH 7.50 |
