2GSD
NAD-dependent formate dehydrogenase from bacterium Moraxella sp.C2 in complex with NAD and azide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.450, 66.500, 75.550 |
| Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
| Resolution | 73.520 - 1.950 |
| R-factor | 0.14441 |
| Rwork | 0.142 |
| R-free | 0.18385 |
| Structure solution method | MOLREP |
| Starting model (for MR) | 2nad |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.520 | 2.030 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.095 | |
| Number of reflections | 28153 | |
| Completeness [%] | 99.3 | 88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Bis-Tris, 1.8M Ammonium Sulfate, 5mM NAD, 5mM azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
