2GRV
Crystal Structure of LpqW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-30 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 188.572, 312.035, 104.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.21684 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 158.110 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.081 | 0.577 |
| Number of reflections | 115857 | |
| <I/σ(I)> | 17.9 | 2.1 |
| Completeness [%] | 97.1 | 93 |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20-24% PEG 4000, 8-16% 2-propanol, 0.1M sodium citrate, 10mM DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
