2GRP
Crystal Structure of human RanGAP1-Ubc9-Y87A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 1 |
Unit cell lengths | 39.429, 45.092, 60.871 |
Unit cell angles | 71.08, 71.11, 75.02 |
Refinement procedure
Resolution | 19.800 - 2.050 |
Rwork | 0.191 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.050 | 0.166 |
Number of reflections | 22558 | |
<I/σ(I)> | 16.8 | |
Completeness [%] | 96.2 | 89.1 |
Redundancy | 2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |