2GRO
Crystal Structure of human RanGAP1-Ubc9-N85Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.295, 44.785, 61.027 |
| Unit cell angles | 72.58, 72.06, 75.45 |
Refinement procedure
| Resolution | 19.980 - 1.700 |
| Rwork | 0.185 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.147 |
| Number of reflections | 39181 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 96.2 | 93.1 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
