2GRK
Crystal structure of ectromelia virus EVM1 chemokine binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.200, 55.800, 85.800 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.600 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cq3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.112 | 0.426 |
| Number of reflections | 13573 | |
| <I/σ(I)> | 9.547 | 2.36 |
| Completeness [%] | 97.6 | 96.4 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 293 | 2 M ammonium sulfate, 3% ethylene glycol, 0.1 M cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K, pH 6.20 |
