2GRH

M37V mutant of Scapharca dimeric hemoglobin, with CO bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-08-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	93.040, 43.930, 83.440
Unit cell angles	90.00, 121.95, 90.00

Refinement procedure

Resolution	30.610 - 1.500
R-factor	0.186
Rwork	0.186
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sdh
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	43789
<I/σ(I)>	17.6
Completeness [%]	94.8	90.2
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	7.5	298	PHOSPHATE BUFFER, pH 7.50, SMALL TUBES, temperature 298K