2GR2
Crystal structure of Ferredoxin reductase, BphA4 (oxidized form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 98.683, 98.683, 172.572 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.310 - 1.850 |
R-factor | 0.20089 |
Rwork | 0.199 |
R-free | 0.23742 |
Structure solution method | Difference Fourier |
RMSD bond length | 0.015 |
RMSD bond angle | 1.569 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.087 | 0.517 |
Number of reflections | 42769 | |
<I/σ(I)> | 22 | 3.7 |
Completeness [%] | 98.7 | 92.3 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 2M Sodium formate, 0.1M Sodium acetate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |