2GQ8

Structure of SYE1, an OYE homologue from S. ondeidensis, in complex with p-hydroxyacetophenone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-02
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	48.558, 83.675, 87.485
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.172
Rwork	0.161
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.530
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.750
High resolution limit [Å]	1.700	1.700
Number of reflections	39223
Completeness [%]	99.7	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.8 M ammonium sulfate, 2% PEG 400, 0.1 % bog, 0.1 tris-hcl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K