2GQ7
Crystal structure of an RNA racemate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-31 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.805 |
Spacegroup name | P -1 |
Unit cell lengths | 26.665, 37.917, 45.419 |
Unit cell angles | 112.58, 100.94, 93.29 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.231 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NDB entry AR0012 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.208 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 20724 | |
<I/σ(I)> | 17 | 2.9 |
Completeness [%] | 98.0 | 97 |
Redundancy | 16 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 7% PEG 400, 0.1 M CaCl2, 50 mM HEPES , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K, temperature 298K, temperature 287K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 400 | ||
6 | 1 | 2 | CaCl2 | ||
7 | 1 | 2 | H2O |