2GQ5
Crystal structure of an RNA racemate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.803 |
| Spacegroup name | P -1 |
| Unit cell lengths | 26.426, 37.952, 45.198 |
| Unit cell angles | 113.16, 100.27, 93.52 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.252 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB entry AR0012 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.472 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 28908 | |
| <I/σ(I)> | 14 | 2.5 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 6.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 7% PEG 400, 0.1 M CaCl2, 50 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | CaCl2 | ||
| 7 | 1 | 2 | H2O |
