2GQ1
Crystal Structure of Recombinant Type I Fructose-1,6-bisphosphatase from Escherichia coli Complexed with Sulfate Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 45.590, 81.270, 170.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.040 - 1.450 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CNQ without ligands waters and residues 52-72 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.040 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.069 | 0.293 |
| Number of reflections | 56471 | |
| <I/σ(I)> | 13.3 | 5.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.95 | 6.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 295 | 2.0 M Amonium Sulfate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
