2GQ0

Crystal Structure of the Middle Domain of HtpG, the E. coli Hsp90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97959
Spacegroup name	P 1 21 1
Unit cell lengths	31.567, 149.167, 60.224
Unit cell angles	90.00, 99.01, 90.00

Refinement procedure

Resolution	19.830 - 1.900
R-factor	0.17725
Rwork	0.175
R-free	0.21438
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk7
RMSD bond length	0.013
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.107	0.344
Number of reflections	42074
<I/σ(I)>	23	4.9
Completeness [%]	97.7	98.1
Redundancy		3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M SODIUM ACETATE TRIHYDRATE, 0.1 M TRIS PH 8.5, 27-32% PEG 4000 OR PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M SODIUM ACETATE TRIHYDRATE, 0.1 M TRIS PH 8.5, 27-32% PEG 4000 OR PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K