2GPS
Crystal Structure of the Biotin Carboxylase Subunit, E23R mutant, of Acetyl-CoA Carboxylase from Escherichia coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.926, 92.619, 86.598 |
| Unit cell angles | 90.00, 98.84, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.20551 |
| Rwork | 0.201 |
| R-free | 0.25979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.468 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.064 | 0.300 |
| Number of reflections | 23691 | |
| <I/σ(I)> | 16.1503 | 3.131 |
| Completeness [%] | 97.7 | 90.7 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 0.1 M magnesium formate, 14% (w/v) PEG3350, 8% (v/v) glycerol, and 20 mM calcium chloride, pH 8.5, VAPOR DIFFUSION, temperature 294K |
