2GP1
Bacteriophage HK97 Prohead II crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2001-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 706.940, 706.940, 706.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 5.200 |
| R-factor | 0.41 |
| Rwork | 0.410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1if0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RAVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 5.320 |
| High resolution limit [Å] | 5.200 | 5.200 |
| Rmerge | 0.178 | 0.366 |
| Number of reflections | 373697 | |
| <I/σ(I)> | 3.7 | 1.7 |
| Completeness [%] | 83.9 | 30.1 |
| Redundancy | 5.4 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes, 0.2M CaCl2, 2-3% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
