2GOU

Structure of wild type, oxidized SYE1, an OYE homologue from S. oneidensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-02
Detector	MAR CCD 165 mm
Wavelength(s)	0.8034
Spacegroup name	P 21 21 21
Unit cell lengths	48.454, 83.600, 87.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.168
Rwork	0.157
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	2.210
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.430
High resolution limit [Å]	1.400	1.400
Number of reflections	69196
Completeness [%]	99.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.8M ammonium sulfate, 2% peg400, 0.1% bog, 0.1M tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K