2GOJ
The crystal structure of the enzyme Fe-superoxide dismutase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.402, 78.417, 88.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.620 - 2.000 |
| R-factor | 0.15769 |
| Rwork | 0.156 |
| R-free | 0.19389 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isa |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.107 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.620 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 25131 | |
| <I/σ(I)> | 7.5 | 2 |
| Completeness [%] | 95.3 | 73.3 |
| Redundancy | 6.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25.5% PEG 4000 (w/v), 0.17 M Sodium Acetate, 0.085 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
