2GNX

X-ray structure of a hypothetical protein from Mouse Mm.209172

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97182
Spacegroup name	C 2 2 21
Unit cell lengths	82.687, 198.962, 67.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.970 - 2.450
Rwork	0.281
R-free	0.32200
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.966	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.070	0.682
Number of reflections	35469
<I/σ(I)>	12.896	1.697
Completeness [%]	91.8	71.4
Redundancy	9.4	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, 0.005 MES PH 8.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (0.003 SODIUM CHLORIDE, 0.10 M HEPES PH 7.5) Crystals cryo-protected with Fomblin followed by Paratone N, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP		293	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, 0.005 MES PH 8.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (0.003 SODIUM CHLORIDE, 0.10 M HEPES PH 7.5) Crystals cryo-protected with Fomblin followed by Paratone N, vapor diffusion, hanging drop, temperature 293K