2GNK

GLNK, A SIGNAL PROTEIN FROM E. COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 63
Unit cell lengths	57.450, 57.450, 54.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.21
Rwork	0.210
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.590
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.01)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.039	0.204
Total number of observations	44483 *
Number of reflections	6913
Completeness [%]	99.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	277 *	30% MPD, 0.1M NAAC, PH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-13.3 (mg/ml)
2	1	reservoir	MPD	30 (%)
3	1	reservoir	sodium acetate	0.1 (M)	pH6.5
4	1	drop	ATP