2GN0
Crystal structure of dimeric biodegradative threonine deaminase (TdcB) from Salmonella typhimurium at 1.7 A resolution (Triclinic form with one complete subunit built in alternate conformation)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.675, 76.827, 78.501 |
| Unit cell angles | 66.12, 89.16, 77.08 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.22154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Biosynthetic threonine deaminase (1TDJ) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.426 |
| Number of reflections | 128680 | |
| <I/σ(I)> | 26.58 | 2.76 |
| Completeness [%] | 93.1 | 86.9 |
| Redundancy | 15.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M citrate buffer pH 6.0, 20% PEG 3350 and 15% t-butanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
