2GMQ
Crystal structure of protein EF0006 from Enterococcus faecalis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929, 0.97940 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 83.122, 83.400, 83.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.760 |
| R-factor | 0.19194 |
| Rwork | 0.191 |
| R-free | 0.21663 |
| Structure solution method | MAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.025 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.093 | 0.635 |
| Number of reflections | 28088 | |
| <I/σ(I)> | 32.09 | 1.82 |
| Completeness [%] | 95.7 | 90.7 |
| Redundancy | 7.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 289 | 20% PEG 3350, 0.2 M CaCl2, 0.1 M Tris, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
