2GMN
Crystal structure of BJP-1, a subclass B3 metallo-beta-lactamase of Bradyrhizobium japonicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.281302 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.501, 44.773, 76.966 |
| Unit cell angles | 78.92, 89.51, 61.91 |
Refinement procedure
| Resolution | 38.580 - 1.400 |
| R-factor | 0.1797 |
| Rwork | 0.176 |
| R-free | 0.21322 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.163 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.576 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 87661 | |
| Completeness [%] | 91.0 | 77.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1M Tris, 0.4M Na acetate, 40% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
