2GMM

Metal-free (apo) P. angolensis seed lectin in complex with Man-alpha(1-2)Man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.8123
Spacegroup name	P 21 21 21
Unit cell lengths	58.706, 62.587, 129.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.150
R-factor	0.188
Rwork	0.185
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	metal-free PAL
RMSD bond length	0.006
RMSD bond angle	1.490
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	26425
Completeness [%]	98.8	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	10% PEG 6000, 20% MPD, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K