2GML

Crystal Structure of Catalytic Domain of E.coli RluF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792, 0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	65.714, 65.714, 215.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.600
Rwork	0.225
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	27726
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	2M ammonium sulfate, 0.2M potassium thiocyanate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K