2GMJ
Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 154.839, 154.839, 130.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 2.600 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gmh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 38797 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 78.9 | 39.6 |
| Redundancy | 3.3 | 1.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Tertiary-butanol, PEG400, Calcium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
