2GLZ

Crystal structure of a formylmethanofuran dehydrogenase subunit e-like protein (dhaf_2992) from desulfitobacterium hafniense dcb-2 at 1.45 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97905
Spacegroup name	P 21 21 21
Unit cell lengths	46.425, 84.791, 100.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.200 - 1.450
R-factor	0.173
Rwork	0.171
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.546
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.894	29.890	1.490
High resolution limit [Å]	1.450	6.480	1.450
Rmerge	0.071	0.045	0.729
Total number of observations		5688	24112
Number of reflections	71200	870	5186
<I/σ(I)>	5.8	12.2	1
Completeness [%]	99.9	94.8	99.9
Redundancy	7.4	6.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	5.8	277	0.2M MgCl2, 20.0% PEG-3350, No Buffer, pH 5.8, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K