2GLQ
X-ray structure of human alkaline phosphatase in complex with strontium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 200 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.090, 115.299, 107.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.990 - 1.600 |
| R-factor | 0.14991 |
| Rwork | 0.148 |
| R-free | 0.18813 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.751 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 72276 | |
| <I/σ(I)> | 19.25 | 2.88 |
| Completeness [%] | 99.0 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Crystals were grown at 19 (2) using sitting drop vapor diffusion, with protein concentration of 10mg/ml, in the presence of 10mM p-nitrophenyl phosphate. Crystals were obtained from 12% PEG 3350, 100mM sodium cacodylate pH 6.5, 1mM zinc chloride, 2mM magnesium acetate, and 2mM strontium chloride over a period of five months, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
