2GLI
FIVE-FINGER GLI/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 149.000, 50.200, 45.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.600 |
| R-factor | 0.228 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.070 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.067 * |
| Total number of observations | 28039 * |
| Number of reflections | 10856 |
| Completeness [%] | 97.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | BIS-TRIS-PROPANE_HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | drop | DNA | 0.6 (mM) | |
| 3 | 1 | reservoir | 60-100 (mM) | ||
| 4 | 1 | reservoir | PEG400 | 20-25 (%) | |
| 5 | 1 | reservoir | BTP-HCl | 50 (mM) | pH7.0 |
