2GLF

Crystal structure of Aminipeptidase (M18 family) from Thermotoga Maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 3
Unit cell lengths	191.225, 191.225, 191.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.800
R-factor	0.168
Rwork	0.168
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	51206
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05M NaCl, 7% EtOH, 0.1M MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K