2GL2
Crystal structure of the tetra mutant (T66G,R67G,F68G,Y69G) of bacterial adhesin FadA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 61 |
| Unit cell lengths | 59.168, 59.168, 188.249 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.200 - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.232 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AVR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.889 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.560 |
| Number of reflections | 11518 | 632 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 89.4 | 48.5 |
| Redundancy | 4.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 295 | Sodium acetate, beta-octyl glucoside, pH 4.6, VAPOR DIFFUSION, temperature 295K |
