2GKT
Crystal structure of the P14'-Ala32 variant of the N-terminally truncated OMTKY3-del(1-5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.049, 34.692, 25.795 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 25.580 - 1.230 |
| R-factor | 0.13183 |
| Rwork | 0.130 |
| R-free | 0.15067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.895 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.270 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Number of reflections | 11526 | |
| Completeness [%] | 97.7 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES-Na pH 7.5, 10% v/v isopropanol, 20% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
