2GKS
Crystal Structure of the Bi-functional ATP Sulfurylase-APS Kinase from Aquifex aeolicus, a Chemolithotrophic Thermophile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.228, 67.283, 108.728 |
| Unit cell angles | 90.00, 105.43, 90.00 |
Refinement procedure
| Resolution | 19.620 - 2.310 |
| R-factor | 0.19821 |
| Rwork | 0.196 |
| R-free | 0.23979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i2d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.235 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 | 0.328 |
| Number of reflections | 49813 | |
| <I/σ(I)> | 4.01 | |
| Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 40% ethanol, 1.0% PEG 6000, 50 mM NaAc, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
