2GKM
Crystal structure of Mycobacterium tuberculosis trHbN TyrB10Phe mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-13 |
| Wavelength(s) | 0.843 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.278, 61.932, 90.291 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.100 - 1.731 |
| R-factor | 0.19466 |
| Rwork | 0.193 |
| R-free | 0.23317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.247 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.100 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.036 | 0.193 |
| Number of reflections | 27177 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 97.1 | 82 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.8 | 277 | sodium dihydrogen phosphate + di-potassium hydrogen phosphate 1.8 M, pH 7.8, VAPOR DIFFUSION, temperature 277K |
