2GK4
The Crystal Structure of the DNA/Pantothenate Metabolism Flavoprotein from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.118, 64.594, 92.839 |
| Unit cell angles | 90.00, 93.14, 90.00 |
Refinement procedure
| Resolution | 37.660 - 1.830 |
| R-factor | 0.18334 |
| Rwork | 0.178 |
| R-free | 0.22945 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.371 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.660 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.109 | 0.427 |
| Number of reflections | 40925 | |
| <I/σ(I)> | 9.3 | 1.96 |
| Completeness [%] | 98.3 | 80.9 |
| Redundancy | 8.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 100 mM Tris pH 8.5, 30 % PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
