2GK3

Cytoplasmic Protein STM3548 from Salmonella typhimurium

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-28
Detector	CUSTOM-MADE
Wavelength(s)	0.979809, 0.979522
Spacegroup name	P 21 21 2
Unit cell lengths	182.892, 79.367, 115.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	115.470 - 2.250
R-factor	0.17967
Rwork	0.177
R-free	0.22643
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.417
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.470	2.340
High resolution limit [Å]	2.250	2.250
Number of reflections	72006
Completeness [%]	93.9	61.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris, pH 8.5, 1.2M tri-sodium citrate dehydrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K