2GK2
Crystal structure of the N terminal domain of human CEACAM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 |
| Unit cell lengths | 86.200, 86.200, 62.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.537 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.736 |
| Number of reflections | 13366 | |
| <I/σ(I)> | 38.8 | 2 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 10.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 16% (w/v) polyethylene glycol mono methyl ether 2000, 100 mM Tris, and 5 mM nickel chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
