2GK0
Structure of Catalytic Elimination Antibody 13G5 from a twinned crystal in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.875, 84.619, 150.074 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.450 |
| R-factor | 0.2357 |
| Rwork | 0.191 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gjz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 30830 | |
| <I/σ(I)> | 14.4 | 1.8 |
| Completeness [%] | 98.0 | 92.9 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | PEG 4000, 0.3M NaHCOO, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
