2GJZ
Structure of Catalytic Elimination Antibody 13G5 from a crystal in space group P2(1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.209, 73.495, 130.475 |
| Unit cell angles | 90.00, 96.33, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.650 |
| R-factor | 0.21494 |
| Rwork | 0.213 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hin |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.431 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.710 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 27894 | |
| <I/σ(I)> | 13.9 | 1.8 |
| Completeness [%] | 97.5 | 91.8 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 8000, 0.1M MES, 0.2M Zn(OAc)2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
