2GJX
Crystallographic structure of human beta-Hexosaminidase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1158 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 321.091, 110.536, 129.668 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 39.870 - 2.800 |
| R-factor | 0.26965 |
| Rwork | 0.269 |
| R-free | 0.28822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.795 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.750 |
| Number of reflections | 111512 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.7 | 2.08 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 13% pEG 8000, 0.1M NaAcetate, 0.2M thiocyanate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
