2GIZ
Structural and functional analysis of Natrin, a member of crisp-3 family blocks a variety of ion channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 79 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.550, 58.910, 86.870 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 21.290 - 1.680 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xx5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.573 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.290 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.052 | 0.287 |
| Number of reflections | 50171 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.98 | 4.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 30% PEGmme 2000, 0.15M KSCN, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
