2GIT
Human Class I MHC HLA-A2 in complex with the modified HTLV-1 TAX (Y5K-4-[3-Indolyl]-butyric acid) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.384, 62.709, 74.773 |
| Unit cell angles | 82.00, 76.22, 78.18 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.18191 |
| Rwork | 0.180 |
| R-free | 0.21947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2av1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.729 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.087 | 0.239 |
| Number of reflections | 87035 | |
| <I/σ(I)> | 14 | 3.13 |
| Completeness [%] | 91.8 | 66.2 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG3350 24%, MES 0.025M, HCOOH 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
