2GIE
HincII bound to cognate DNA GTTAAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 67.243, 175.472, 253.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.238 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tx3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.095 | 0.952 |
| Number of reflections | 46277 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 97.4 | 95.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | 25% PEG 4K, 0.15 M NaCl, 0.1 M citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4K | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | citrate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 4K | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | citrate | ||
| 8 | 1 | 2 | H2O |
