2GI7
Crystal structure of human platelet Glycoprotein VI (GPVI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.057, 45.286, 75.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.520 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 15739 | |
| <I/σ(I)> | 6.6 | 2.1 |
| Completeness [%] | 99.3 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 293 | 0.9M ammonium sulfate, 8% MPD, 20% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.40 |
