2GHB
Thermotoga maritima maltotriose binding protein, ligand free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.040, 106.230, 186.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.190 - 2.100 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.309 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.082 | 0.419 |
Number of reflections | 84588 | 10237 |
<I/σ(I)> | 19.05 | 4.57 |
Completeness [%] | 96.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 290 | 0.2M Magnesium Chloride Hexahydrate, 0.1M pH5.5 Bis-Tris, 25% PEG 2000, VAPOR DIFFUSION, temperature 290K |