2GHA
Thermotoga maritima maltotriose binding protein bound with maltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Collection date | 2004-12-15 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.728, 55.510, 93.556 |
| Unit cell angles | 96.89, 93.08, 102.05 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.332 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.067 | 0.874 |
| Number of reflections | 97803 | 9196 |
| <I/σ(I)> | 7.8 | 1.4 |
| Completeness [%] | 86.8 | 81.4 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 290 | 0.2M Magnesium Chloride Hexahydrate, 0.1M pH5.5 Bis-Tris, 25% w/v PEG 3350, VAPOR DIFFUSION, temperature 290K |
