2GH8
X-ray structure of a native calicivirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-11 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 457.553, 457.553, 457.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRYOEM STRUCTURE OF SAN MIGUEL SEA LION VIRUS AT A RESOLUTION OF 19 ANGSTROM |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.414 |
| Data scaling software | SCALEPACK |
| Phasing software | RAVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.122 | 0.469 |
| Number of reflections | 248269 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 95.8 | 84.7 |
| Redundancy | 3.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 295 | 0.8M ammonium sulfate, 1M lithium sulfate, 0.1M trisodium citrate dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.60 |
