2GGZ

Crystal Structure of Human Guanylate Cyclase Activating Protein-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000
Spacegroup name	P 43
Unit cell lengths	88.190, 88.190, 71.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.430 - 3.000
R-factor	0.256
Rwork	0.256
R-free	0.29000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.600
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	10427
Completeness [%]	94.4	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1 M MES, 5% PEG 6000, 5% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1 M MES, 5% PEG 6000, 5% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K