2GGU
crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with maltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.730, 108.930, 55.840 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.225 |
| Rwork | 0.221 |
| R-free | 0.24800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pwb |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.052 | 0.300 |
| Number of reflections | 56684 | 5612 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 12% PEG 8000, 150mM NaCl, 10mM CaCl2, 100mM HEPES , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
