2GGS
crystal structure of hypothetical dTDP-4-dehydrorhamnose reductase from sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.944, 40.683, 103.140 |
| Unit cell angles | 90.00, 116.18, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.700 |
| R-factor | 0.21009 |
| Rwork | 0.208 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n2s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.716 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 62990 | |
| Completeness [%] | 98.9 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 14% PEG 3350, 0.1M KI, 20% BENZAMIDINE, 20mM TRIS-HCL, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
