2GGO
Crystal Structure of glucose-1-phosphate thymidylyltransferase from Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-03 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.999, 122.999, 94.884 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 1.800 |
| R-factor | 0.2242 |
| Rwork | 0.222 |
| R-free | 0.26749 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.052 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | |
| Number of reflections | 48014 | |
| Completeness [%] | 96.6 | 90.2 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% PEG 3350, 0.5M CsCl, 20mM TRIS-HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
